Dear Lisa, because we know nothing about our object: protein is membrane
or globular? Is there known structures of homologs?
What buffer do you use?
17.02.2014 13:57, LISA пишет:
> Dear All,
>
> My proein is polymerzated and elutate in the void volum when runnning
> gel filtration Superdex 200. I can get a small crystal after a lot of
> optimization. But the resolution is still very low (about 8A ). I try
> to find the sites involved in polymerization. Is there some software
> to predicat the amino acids which are involved in protein
> polymerization? And All suggestion to the crystal optimization are
> welcome. Thank you.
>
> Best Regards,
>
> Lisa
--
Eugene Osipov
Junior Research Scientist
Laboratory of Enzyme Engineering
A.N. Bach Institute of Biochemistry
Russian Academy of Sciences
Leninsky pr. 33, 119071 Moscow, Russia
e-mail: [log in to unmask]
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