Hi Juha,
This is the normal behaviour: movie processing is only done for a single
iteration and continues from the last iteration of a "normal" refinement.
Just wait until that last iteration finishes and re-run post processing on
the resulting until.mrc maps.
HTH,
S
> Hello,
>
>
> I have refined my data using particles picked from the average of all
> frames. Then I have extracted the particles from the individual frames as
> explained in the wiki. Now I am trying to continue the refinement using
> these. My problem is that the refinement does not proceed, but rather
> enters the final round immediately.
>
>
> This is the command line:
>
>
> mpirun -np 8 `which relion_refine_mpi` --o Refine3D/run_movie_f7a2_ct10
> --continue Refine3D/run1_it010_optimiser.star --realign_movie_frames
> data_movie.star --movie_frames_running_avg 2 --sigma_ang 0.5 --sigma_off
> 0.5 --flatten_solvent --oversampling 1 --auto_local_healpix_order 6 --norm
> --scale --j 8
>
>
> And the output:
>
>
> Expanding current model for movie frames...
>
> Performing local angular searches! Lowering max_nr_pool from 8 to 1!
>
> Auto-refine: Iteration= 11
>
> Auto-refine: Resolution= 11.1704 (no gain for 0 iter)
>
> Auto-refine: Changes in angles= 999 degrees; and in offsets= 999 pixels
> (no gain for 0 iter)
>
> Auto-refine: Refinement has converged, entering last iteration where two
> halves will be combined...
>
>
> What am I doing wrong?
>
>
> Best regards,
>
> Juha
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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