No real need, some folks do it like that.
However, you'll need to fish out your protein from more junk and you may have more proteolytic degradation.
Also, you will need more detergent compared to "membrane-only" extraction, especially when using mild detergents.
bert
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From: CCP4 bulletin board [[log in to unmask]] on behalf of Theresa Hsu [[log in to unmask]]
Sent: Monday, January 06, 2014 9:56 PM
To: [log in to unmask]
Subject: [ccp4bb] Membrane fractionation
Dear crystallographers
When we purify membrane proteins, we often fractionate the E. coli cell membrane by ultracentrifugation. Is there a need for this step instead of solubilizing the whole cell with detergents?
Thank you.
Theresa
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