Dear Jens, Careina
this is not really an *advantage*, but rather a *convenience*. You can still use big crystals if you'd like to, but as they usually never survive more than one shot (few femtosec), you'd need a lot of these bigger crystals to collect a full data.
And yes, I would also highly recommend XFELs!
Cheers, Leo
On Dec 10, 2013, at 4:36 AM, Jens Kaiser <[log in to unmask]> wrote:
> Careina,
> If your target is interesting enough, try to reproduce the small
> crystals in batch and apply for FELS time. Small crystals are actually
> an advantage there.
>
> Cheers,
>
> Jens
>
>
> On Wed, 2013-12-04 at 21:41 -0800, Careina Edgooms wrote:
>> Hi all
>>
>>
>> Any advice on how to get bigger crystals from conditions that give
>> showers of tiny crystals? I am getting small pretty looking individual
>> crystals but they are too small and they don't seem to grow. In fact,
>> in some instances if left for a couple of days they actually dissolve.
>> I have fiddled around with mother liquor volume, protein concentration
>> as well as drop volume (I am using hanging drop method) but none seem
>> to make any difference and I always get the same tiny crystals. I
>> think I might try microseeding but I haven't tried that yet.
>>
>>
>> Any suggestions or tricks would be welcome
>> Careina.
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