Hi Tomas,
Whether the peak can be assigned would depend on the size of the perturbation. If you peaks are reasonably far from each other, you could assign a different shiftlist to each spectrum and then the problem can be avoided.
Shift list designation is found under Spectra:Experiments
Hope this helps
Regards
Simon
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Simon P Skinner
Protein Chemistry Group
Leiden Institute of Chemistry, Universiteit Leiden
Phone: +31 71 527 6089 / Fax: +31 71 527 4349
E-mail : [log in to unmask]
________________________________
Van: CcpNmr software mailing list namens Kroupa Tomas (980)
Verzonden: do 8/22/2013 9:41
Aan: [log in to unmask]
Onderwerp: Ccpnmr Analysis - Chemical shift perturbance
Dear Sir or Madam,
I encounter a problem with Ccpnmr Analysis when I use it to assign spectra from nmr titration experiments.
I am probably doing something wrong, but I could not find any recommendation in the manual or on the internet.
When I try to assign spectra from HN HSQC experiments, I pick a peak, but I cannot find right residue in the Assigment Panel (even if i use double tolerances).
I tried also Copy Assignments, but most of the peaks were not assigned or were assigned partially (only in hydrogen domain) and I could not correct it manualy because it did not work.
Can you please give me some advice?
Thank you for your time.
Sincerely
Tomas Kroupa
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