Dear Fei,
in addition, take a look here:
https://www.researchgate.net/profile/Fulvio_Saccoccia/contributions/?ev=prf_act
and download my tutorial on XDS if you want.
Bye
Il 04/07/2013 18:26, Fei Li ha scritto:
> Dear all,
>
> I recently collected some 10 degree wedges of data on a very small
> membrane protein. The data processing so far is not working well. XDS
> is the only software that process my data and give a space group and
> cell parameters. However, I ended up with a merged dataset with low
> completeness at low resolution. My data is not overloaded. This is a
> very small protein shot with 5um mini-beam. I checked the FRAME.cbf
> file and looks like XDS is not picking the spots at low resolution
> correctly. It missed a good portion of my low res spots in every
> datasets I checked and picked more at high res, which are weaker. Then
> the indexing step will reject most of my spots for "too far away from
> ideal position". imosflm was able to pick the spots correctly, but
> does not index. I can send the FRAME.cbf file and the imosflm picture
> if anyone thinks that will he useful to see. Any suggestion on how to
> make XDS, or imosflm, correctly working for me is greatly appreciated.
> Thanks a lot!
>
> Best regards,
>
> Fei
>
>
> Fei LI
> Graduate Assistant
> 310 Biochemistry Building
> Department of Biochemistry and Molecular Biology
> Michigan State University
> East Lansing, MI
> 48824
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