It turns out that these images contain two distinct lattices, separated by a rotation of about 5 degrees. Using the development version of iMosflm the two lattices can be easily indexed and integrated. However, the data is quite incomplete due to severe radiation damage.
Andrew
On 2 Jul 2013, at 15:43, RHYS GRINTER <[log in to unmask]> wrote:
> Hi All,
>
> I collected some data on the weekend on forked crystal, I collected data on this crystal at the base before the crystal split into two.
> The crystal didn't stand up well to the radiation damage so I shot a number of places along the crystal and got maybe 45 degrees of good data per position. Auto-processing failed on all but one data set, this dataset processed to 3.99 A, but only with around 80% completeness. However looking at the diffraction images I see spots in the first 45 degrees to at least 3.2 angstroms.
>
> I tried quickly to manually process in mosflm, but I noticed that many of the spots appear to be in fact made up to two very closely located spots. This data was collected at a micro-focus station so it was impossible to tell this without careful analysis of the spots. I guess these spots are an indication that the lattice was splitting even at this point.
>
> As a relative novice at data processing, I'm wondering if this kind of data is processable and if so what is the best strategy (or if I should just get back to the bench and grow some more crystals) and program to use?
>
> Cheers,
>
> Rhys
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