Hi all.
  I don't have any experience with Clontech and Fisher 
resins, but about the GE one I faced the same problem as 
Sebastiano indicated.
But I have to say that the problem was not general, but 
protein (or family of protein) related: the low binding 
depends on the oligomerization state of the chimeric 
protein. When it behaves as dimer (GST dimerizes) it's 
fine, when the oligomeric state increases (in my case 
hexamer) I really can't manage to purify the protein on 
GST sepharose. GST not exposed...I guess...

I'm waiting for the replies Mirek's question...I'm 
interested too.

Ciao,
  Barbara




On Wed, 5 Jun 2013 09:32:28 +0200
  Sebastiano Pasqualato <[log in to unmask]> 
wrote:
> 
> Hi Mirek, hi all,
> I'm also very interested in the topic, so please keep me 
>up with the replies, or make sure to post a summary, 
>please.
> 
> In addition to the price, the problem we're facing with 
>GSH-beads from GE (although we haven't tried others yet) 
>is that we can't manage to deplete our lysates.
> We are always left with a large amount of unbound 
>GST-tagged protein in the flow through, that is 
>eventually captured by a second, third and sometime 
>fourth  incubation with fresh beads.
> Using larger beads volume won't help.
> Has anybody faced and/or overcame this problem?
> 
> Thanks a lot in advance,
> ciao,
> 
> Sebastiano
> 
> 
> On Jun 4, 2013, at 8:54 PM, "Cygler, Miroslaw" 
><[log in to unmask]> wrote:
> 
>> Hi,
>> I would like to ask the bb faithful for their experience 
>>with the glutathione affinity resins. We have been using 
>>so far the Glutathione Sepharose fast flow from GE but 
>>the price is getting steeper. We found Glutathione 
>>Superflow resin from Clontech to be significantly less 
>>expensive and Glutathione agarose from Fisher somewhere 
>>in-between. We have no experience with the latter two 
>>resins and I wonder what is the experience of other 
>>people with these resins? Do they have decent binding 
>>capacity? Can they be efficiently regenerated or are they 
>>a single use only?
>> Thanks for your help,
>> 
>> Mirek
>> 
>> 
>> 
> 
> -- 
> Sebastiano Pasqualato, PhD
> Crystallography Unit
> Department of Experimental Oncology
> European Institute of Oncology
> IFOM-IEO Campus
> via Adamello, 16
> 20139 - Milano
> Italy
> 
> tel +39 02 9437 5167
> fax +39 02 9437 5990
> 
> 
> 
> 
> 
> 

Barbara Giabbai

Elettra-Sincrotrone Trieste S.C.p.A.
SS 14 - km 163,5 AREA Science Park
34149 Basovizza, Trieste ITALY
Office:   +39 040 375 8840
Lab:      +39 040 375 8537