Hello,
I have been having some weird results with the follow shift changes
tool. Basically, I have picked peaks, and my experiments are in an NMR
series by concentration. However, the Kd value which I get after fitting
is negative and meaningless (all of the peaks have identical Kd values
to 2 decimal places.). A screenshot of my setup is attached.
If instead I setup an NMR series by ligand ratio, the analysis works and
I get reasonable Kd values. Is this a bug or am I missing something with
the setup?
Thanks!
David
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