Hi Juha,
Yes, if yu give the data.star file from the 1st round, the refined X and Y
origin offsets will be taken to the next round. If you then set the
translational search range to zero (or a small value), the particles will
no longer move. Note that the stddev on the origin offsets will not
change: as those are calculated w.r.t. the original coordinates in the
micrographs.
Hope that helps,
Sjors
> Hi Sjors and RELION users,
> My data consists of very heterogeneous, elongated particles. I am
trying
> to do 2D classification in two stages: First, using a large mask, to
approximately center all the particles. Second, to classify only the
middle
> part of the particles, using a smaller mask to exclude noise and the
more
> variable ends.
> While the first step works ok, I am wondering if I can give the output data
> file from the first classification as an input for the second. And more
specifically, if the updated origins from the first classification are
treated as a priori information in the second?
> Best wishes,
> Juha
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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