hello everyone,
i am working with a reductase and my protein got crystallize. however
after 2-4 days the crystal just vanished in drop. i incubated the
protein with ligand (Nadp) and set up the screen. now when i am trying
to set up the trials in and around the condition nothing is coming
except precipitation.besides this the protein forms skin at the edge
of the drop.can i try microseeding from that drop? Has anyone got any
suggestions?
rakesh
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