Dear Uma,
A very complehensive survey of the state-of-the-art in this topic can be found in the following literature resources:
(1)
Garman EF.
Radiation damage in macromolecular crystallography: what is it and why should we care?
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):339-51.
(2)
The January 2013 issue of the Journal of Synchrotron Radiation featuring a special section with papers presented at last year's Intl workshop on X-ray damage of biological crystalline samples.
The annotated editorial by Garman and Weik in that issue is also very helpful.
Best regards
Savvas
On 13 Feb 2013, at 23:38, Uma Ratu wrote:
> Dear All:
>
> I plan to use X-ray crystallography method to study the S-nitrosylated protein structure.
>
> The native protein crystals diffracted to 2A with synchrontron. I now have the crystals of S-ntrosylated protein.
>
> Since S-NO moiety appears to be unstable to synchrotron radiation, could you advice / comments on the stratage on the data collection of S-nitrosylated protein crystals?
>
> The protein crystals did not diffract well with in house X-ray.
>
> Thank you for your comments.
>
> Uma
>
|