Side note,
Has anyone tried this in a non-E. coli expression system?
Thanks,
Lori
Lori Horton, Ph.D.
Koehler Lab
[log in to unmask]<mailto:[log in to unmask]><mailto:[log in to unmask]>
713-500-5451
________________________________
From: CCP4 bulletin board [[log in to unmask]] On Behalf Of A K [[log in to unmask]]
Sent: Tuesday, February 26, 2013 4:45 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] Additives to bacterial cultures to improve solubility
Mark,
you can try low percentage of ethanol as well, as it increases the expression of some chaperones (can't find the paper right now!).
Alex
On Tue, Feb 26, 2013 at 2:08 AM, Mark J van Raaij <[log in to unmask]<mailto:[log in to unmask]>> wrote:
Dear All,
We are considering trying additives to bacterial cultures to see if they improve solubility of some proteins we express: L-arginine, glycyl-glycine, sorbitol and betaine. I would be glad to receive recommendations and experiences, also if they haven't worked.
Side-question: should we get cell culture-certified compounds, or would "normal", much more economical ones suffice? (i.e. are they likely to contain side-products that would affect bacterial growth?).
Greetings,
Mark
Mark J van Raaij
Lab 20B
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616<tel:%28%2B34%29%2091%20585%204616>
http://www.cnb.csic.es/~mjvanraaij<http://www.cnb.csic.es/%7Emjvanraaij>
--
David J. Leibly
Graduate Student, Yeates Lab
Dept. Chem & Biochem
Univ. of California, Los Angeles
Boyer Hall 269B
(310) 825-8901
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