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Dear Yuri,
If you have access to mass spec, this should be a straight forward experiment. Find the
reference here.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2291582/pdf/v010p00017.pdf
What was the result in the EllmanĀ“s reaction? Unless you have other reactive cysteines
in your protein protein, you should not see any color if the pair of cysteines on
surface form disulfide.
Anthony
-----------------------------------------------------
Dr. Anthony Addlagatta
Center for Chemical Biology
Indian Institute of Chemical Technology [IICT]
Tarnaka, Hyderabad
AP-500 607, INDIA
Tel:91-40-27191812
Web: https://sites.google.com/site/chembioliict/home/dr-anthony-addlagatta-1
---------- Original Message -----------
From: Yuri Pompeu <[log in to unmask]>
To: [log in to unmask]
Sent: Wed, 6 Feb 2013 16:10:35 +0000
Subject: [ccp4bb] Off Topic- Cystine Detection
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> This message has been scanned by the InterScan for CSC SSM at IICT and found to be
free of known security risks.
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>
> Dear All,
> I am trying to probe the existence of a disulfide bond on the surface of my protein.
> I have attempted EllmanĀ“s and my results were not as clear as I would have hoped for.
> I am not a sulfur/cysteine chemist and would appreciate the advice on what experiments
to try!
> Thanks a bunch
> YAP
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