You might try mercury soaks instead. Might give you 15 minute answers to your questions.
HNY!
Ho
Sent from my iPad
On Dec 26, 2012, at 4:00 PM, CCP4BB automatic digest system <[log in to unmask]> wrote:
> There is 1 message totaling 34 lines in this issue.
>
> Topics of the day:
>
> 1. SeCys usage for SAD
>
> ----------------------------------------------------------------------
>
> Date: Wed, 26 Dec 2012 12:33:02 -0600
> From: Linda Olson <[log in to unmask]>
> Subject: SeCys usage for SAD
>
> Dear All,
>
> I have read a recent paper by Salgado et al about using non-auxotrophic E.coli to incorporate SeCys into recombinant protein for phasing purposes. Does anyone have a source for Selenocysteine? I have also seen a paper by Schaefer et al which uses nitric acid treated elemental Se for a sulfur surrogate to generate Se-labeled protein. Has anyone else tried this? My proteins are rich in Cys and tend to lack Met so the prospect of labeling cys is very attractive.
>
> Thanks,
>
> Linda
> ________________________
> Linda Olson, PhD
> Research Scientist II
> Dept Biochemistry
> Medical College of Wisconsin
> 8701 Watertown Plank Rd
> Milwaukee, WI 53226
>
> phone: 414-955-8545
> fax: 414-456-6510
> _________________________
>
> ------------------------------
>
> End of CCP4BB Digest - 24 Dec 2012 to 26 Dec 2012 (#2012-363)
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