Hi there,
You could try to go for liquid-liquid interface crystallisation (in
capillaries). The minimum volume would be no less than 1.5 microl
protein + 1.5 microl precipitant, i.e. not the volumes used by nanodrop
robots. Crystallisation under oil (or similar liquids) should also have
the same effect (no spreading out).
HTH,
Fred.
On 07/11/12 13:07, Eva Bligt-Lindén wrote:
> Dear ccp4 users,
>
> I have a problem in the crystallization of my target protein. Whenever
> I set up a vapour diffusion experiment, either hanging or sitting
> drops, the drops spread out. The surface tension is completely lost in
> 80-90% of the droplets. Have any one experienced something similar?
> What could be the reason for this strange behaviour? I have tried
> three different commercial screens with 96 condition each and there is
> no difference between the screens. There is no difference between
> manual or robotic setups either. The protein buffer is 40 mM Tris, 2
> mM MgCl2 buffer, pH 7.4. The buffer controls are all ok.
>
> Kind regards,
> Eva
>
> ____________________________________
>
> Eva Bligt-Lindén (M.Sc.)
> PhD student
> Structural Bioinformatics Laboratory
>
> Department of Biosciences,
> Ĺbo Akademi University
> BioCity, Tykistökatu 6A
> FI-20520 Turku
> Finland
>
>
--
Fred. Vellieux (B.Sc., Ph.D., hdr)
IBS / ELMA
41 rue Jules Horowitz
F-38027 Grenoble Cedex 01
Tel: +33 438789605
Fax: +33 438785494
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