Well originally we got these in cacodylate and not much else would diffract.
http://www.pdb.org/pdb/explore/materialsAndMethods.do?structureId=2EPH
But nowadays I have another conditions, which does not require cacodylate and works well with Hepes.
Jürgen
On Nov 9, 2012, at 7:26 AM, Frank von Delft wrote:
> Hi all -
>
> Anybody know
> a) how hazardous is cacodylate?
> b) does it really matter for crystallization screens?
>
> It seems by far the most hazardous component of the standard screens;
> this 2011 paper seems to think so (bizarrely, I can't access it from
> Oxford):
> http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2818.1977.tb01136.x/abstract
>
> and this is site says lethal dose is 0.5-5g/kg:
> http://cameochemicals.noaa.gov/chemical/4468
> meaning 2ml of a 0.1M solution contains 1/10th lethal dose...? (Someone
> should check my maths...) [Coarse screens come mixed 2ml per condition.]
>
>
> Has anybody done careful experiments that showed it really mattered for
> a given crystal -- or even an entire screen?
>
> So I'm inclined to toss it out entirely rather than make crystallization
> screening a "hazardous activity". (We're being subjected to a safety
> review.)
>
>
> Thoughts welcome.
> phx
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-2926
http://lupo.jhsph.edu
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