Hi,
First I'm sorry for my blank message earlier.
Doesn't this depend on the oscillation angle? If those images were
collected using 0 to 1° oscillations, I would assume he has a badly
diffracting protein crystal.
Ganesh
Le 13/11/12 11:34, Tim Gruene a écrit :
> -----BEGIN PGP SIGNED MESSAGE-----
> Hash: SHA1
>
> Dear George,
>
> the images don't look like a large cell to me: on the first image you
> can see spots from ice rings at about 4A and there are only very few
> spots inside that radius, all of which are at beyond 5A.
> I'd say there are traces of MgS04 or CaSO4, the Ca or Mg being
> left-overs from the expression media.
>
> Regards,
> Timd
>
> On 11/13/2012 11:17 AM, George wrote:
>>
>> Dear colleagues,
>>
>>
>>
>> There are some crystallographers with (much) more experience than
>> me.
>>
>> I ‘ve attached few diffraction images which are not (in my opinion)
>> typical salt but not typical protein either.
>>
>> Please let me know you suggestions. Is it worth investigating
>> further those conditions or there are just salts crystals with
>> large unit cell.
>>
>> Attached files:
>>
>> Crystal.jpg (Photo of crystal)
>>
>> dif_0.jpg (Diffraction 900 sec exposure/degree at 0 degrees)
>>
>>
>> dif_90.jpg (Diffraction 900 sec exposure/degree at 90 degrees)
>>
>>
>> dif_180.jpg (Diffraction 900 sec exposure/degree at 180 degrees)
>>
>>
>> dif_270.jpg (Diffraction 900 sec exposure/degree at 270 degrees)
>>
>>
>>
>>
>>
>>
>> protein: 3.3mg/ml in 20 mM TrisHCl pH 8, 150 mM NaCl, 5 mM DTT.
>>
>> Mother liquor: 28%Jefamine, 0,05mM Li2SO4, 10mM Tris pH 8.0
>>
>> sitting drops, 1.2ul protein / 1.2ul mother liquor.
>>
>>
>>
>> Thanks in advance for your help,
>>
>>
>>
>> George Kontopidis
>>
>>
>>
>> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf
>> Of Frank von Delft Sent: Tuesday, November 13, 2012 8:56 AM To:
>> [log in to unmask] Subject: Re: [ccp4bb] Reservoir buffer
>>
>>
>>
>>
>>
>> Acta Cryst. (2005). D61, 490-493 [ doi:10.1107/S0907444905002726
>> <http://dx.doi.org/10.1107/S0907444905002726> ]
>>
>>
>> Expanding screening space through the use of alternative reservoirs
>> in vapor-diffusion experiments
>>
>>
>> J. Newman
>> <http://scripts.iucr.org/cgi-bin/citedin?search_on=name&author_name=Newman,%20J.>
>>
>>
>>
>> Abstract: Setting up vapor-diffusion crystallization experiments
>> against four different reservoir solutions showed that the
>> reservoir solution may have a profound effect on the outcome of a
>> crystallization experiment. This suggests that a facile way to
>> increase crystallization space through screening is not to add more
>> crystallization conditions to the process, but to set up the same
>> conditions over different reservoirs.
>>
>>
>>
>>
>>
>> On 13/11/2012 06:03, Theresa Hsu wrote:
>>
>> Dear all
>>
>> In *initial screening* using vapor diffusion crystallization, does
>> it matter whether the reservoir buffer is also the precipitant in
>> the drop or just a high salt solution like 5 M NaCl?
>>
>> Thank you.
>>
>> Theresa
>>
>>
>>
>>
> - --
> - --
> Dr Tim Gruene
> Institut fuer anorganische Chemie
> Tammannstr. 4
> D-37077 Goettingen
>
> GPG Key ID = A46BEE1A
>
> -----BEGIN PGP SIGNATURE-----
> Version: GnuPG v1.4.12 (GNU/Linux)
> Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/
>
> iD8DBQFQoiIZUxlJ7aRr7hoRAlOtAKD9vm5OgG/GH/SMXv4LwTybKnlU3gCghuVH
> dFhZk5C5gK3fjVG1z00+jzw=
> =VN4A
> -----END PGP SIGNATURE-----
>
|