All is correct, but it is why the cryo protein crystallography was invented (prior and not yet related to synchrotron wide use), However in the case of necessity
capillaries can and should be used. On the attached picture bacterioferritin crystals is mounted in 1.5 mm
glass capillary. Reasons: a. Large crystal b. The 1.5 mm capillary have the thinnest walls.
BTW 1 the capillaries should be siliconised as without this action they are useless.
We still possess know-how technology in the tips of our fingers and maybe it is time to write a retro (memoirs) in one of the relevant journals + small move
BTW 2. All necessary materials can be found in the Hampton Research catalog.
FF
Dr Felix Frolow
Professor of Structural Biology and Biotechnology, Department of Molecular Microbiology and Biotechnology
Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor
e-mail: [log in to unmask]
Tel: ++972-3640-8723
Fax: ++972-3640-9407
Cellular: 0547 459 608
On Nov 13, 2012, at 03:10 , William G. Scott <[log in to unmask]> wrote:
> On Nov 12, 2012, at 8:13 AM, Michael Roberts <[log in to unmask]> wrote:
>
>> Dear All,
>>
>> I would be interested to learn of other crystallographers' experience in their use of glass capillaries for protein crystal growth and X-ray diffraction clarity.
>> There are many types of glass available - quartz, soda glass, borosilicate, etc. Are there specific types which people prefer for best results overall?
>>
>> Best wishes,
>>
>> Michael Roberts
>
> Glass capillaries can be reactive with crystals. Quartz tends to be more neutral and has the advantage of being easier to cut, etc. as well. It does however create a higher background scatter, so the signal to noise ratio of the data collected might suffer a wee bit.
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