Reply-To: | | [log in to unmask][log in to unmask], 12 Oct 2012 10:23:32 -0700558_us-ascii Hi,
Yes, it is worth trying. Nonionic detergents can be good for this.
One example I know of and readily comes to mind is the use of 0.1% NP40 (Noniondet P40) in the stabilization of murine reverse transcriptase during purification (also helps toprevent precipitation), first described in: "Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli.", Roth MJ, Tanese N, Goff SP., J Biol Chem. 1985 Aug 5;260(16):9326-35. http://www.ncbi.nlm.nih.gov/pubmed/2410413 [...][log in to unmask] |