Depends on what you mean by large, but we saw very obvious domain
movement when we soaked a protein (transcription factor TFIIS) into
crystals of RNA Polymerase II (PMIDs 12914699/21346759/22726432). We
recently animated this conformational change, and you can watch it at:
http://www.youtube.com/watch?v=WlMV_l88Lus
...fast forward to about 5 mins 20 second to watch TFIIS binding. And,
er, sorry for the shameless plug ;)
Alan
On 09/10/2012 15:33, WENHE ZHONG wrote:
> Dear CCP4 members,
>
> Recently, I got a ligand soaking structure to clearly show a large
> domain (~100 amino acids) movement compared to the no soaking structure.
> Although there are some reported examples of this enzyme to suggest the
> flexibility of this large domain which is relevant to substrate binding.
> _But it is the first time I can see it happen in crystal soaking
> procedure._ In this case, I am pleased by this result.
>
> My question is, do you have any other example like mine, where domain
> (or loop) movement is observed _*in crystal*_ during ligand _*soaking*_
> procedure? It would be very helpful for me to relate my result to other
> similar examples. Thank you very much.
>
> King regards,
> Wenhe
--
Alan Cheung
Gene Center
Ludwig-Maximilians-University
Feodor-Lynen-Str. 25
81377 Munich
Germany
Phone: +49-89-2180-76845
Fax: +49-89-2180-76999
E-mail: [log in to unmask]
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