Greetings,
I have two issues on which I would like some advice:
1). I know my protein has multiple side chain amines, I can guess what they are in the HSQC. However, I'm curious why when I open "Initialize Root Resonances" popup window, the Sidechain Amine table is not populated. Is there a step I'm missing? The 15N resonances are well within the specified tolerances (0.02 ppm)
2) after I initialize the root resonances, I want to propagate the assignment through a number of standard backbone experiments, e.g. HNCO, HNCA, HNCOCA, etc. When I open the "Pick and Assign from Roots" window, I try to select my root peak list and root peak window, but the only options are <NONE>. Why is my HSQC window and associated peak list not recognized?
Any pointers would be most appreciated.
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