Dear Wojtek,
you can try something of this type to get the EM map converted into an hkl list and mtz file with I's and some sigma I's estimated to 1/10 of the I's (the latter is required for running fittings/refinements etc.).
Best,
Bruno
/usr/local/rave/rave_linux/lx_mapman <<EOF
re m2 name_40-8A.ccp4 ccp4
no m2
uvw
m2
3
1
2
write m2 name_40-8A-uvw.ccp4 ccp4
quit
EOF
/usr/local/ccp4-version/bin/sftools <<EOF
MAPIN name_40-8A-uvw.ccp4 map
MAP2SF
calc q col sigma = col 1 10 /
CALC I col test = rfree(0.05)
WRITE name_40-8A-uvw.fobs col 1 3 2 4
xpl
FOBS
PHASE
write
name_40-8A-uvw.mtz
quit
-----Message d'origine-----
De : CCP4 bulletin board [mailto:[log in to unmask]] De la part de Wojtek Potrzebowski
Envoyé : Friday, July 13, 2012 5:28 PM
À : [log in to unmask]
Objet : [ccp4bb] Rigid body refinement with phenix using EM maps.
Dear all,
I want to refine a model of the complex that consists of rigid subunits using an electron microscopy electron density map.
I've tried to use phenix for this purpose. Thus I have to back-FT the electron density map to a mtz reflection file.
I've experienced problem using sfall for this purpose, but cinvfft has done a job (I can read a resulting file with mtzdmp).
However phenix doesn't read this file properly (data labels box is empty).
Can anyone help me on this, please?
I am looking forward to hearing from you, Wojtek
###########################################################################
Dr. Bruno P. Klaholz
Department of Integrated Structural Biology
Institute of Genetics and of Molecular and Cellular Biology
IGBMC - UMR 7104 - U 964
1, rue Laurent Fries
BP 10142
67404 ILLKIRCH CEDEX
FRANCE
http://www.igbmc.fr/
http://igbmc.fr/Klaholz
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