I can only confirm what Alex said. And the structure was neither a
globin or zyme or psin!
Victor
Quoting aaleshin <[log in to unmask]>:
> I and Victor Lamzin solved our first protein structure (3A
> resolution) in 80-s using pure MIR and a home made (Russian)
> diffractometer...
>
> Alex
>
> On Jun 6, 2012, at 1:42 PM, Boaz Shaanan wrote:
>
>> So if get the gist of the thread right, am I correct in assuming
>> that the last protein structures to be solved strictly by MIR are
>> haemoglobin/myoglobin, lysozyme and chymotrypsin and perhaps one or
>> two more in the late sixties? In which case the answer to the
>> original question about MIR being obsolete, is "yes it is since a
>> long time"?
>>
>> Boaz
>>
>>
>> Boaz Shaanan, Ph.D.
>> Dept. of Life Sciences
>> Ben-Gurion University of the Negev
>> Beer-Sheva 84105
>> Israel
>>
>> E-mail: [log in to unmask]
>> Phone: 972-8-647-2220 Skype: boaz.shaanan
>> Fax: 972-8-647-2992 or 972-8-646-1710
>>
>>
>>
>>
>>
>> ________________________________________
>> From: CCP4 bulletin board [[log in to unmask]] on behalf of Phil
>> Evans [[log in to unmask]]
>> Sent: Wednesday, June 06, 2012 6:04 PM
>> To: [log in to unmask]
>> Subject: Re: [ccp4bb] Fun Question - Is multiple isomorphous
>> replacement an obsolete technique?
>>
>> No they were not useless! I used them
>>
>> (probably better now with cryo data though)
>>
>> Phil
>>
>> On 6 Jun 2012, at 16:02, Dyda wrote:
>>
>>>> I suspect that pure MIR (without anomalous) was always a fiction.
>>>> I doubt that anyone has ever used it. Heavy atoms always give
>>>> an anomalous signal
>>>
>>>> Phil
>>>
>>> I suspect that there was a time when the anomalous signal in data
>>> sets was fictional.
>>> Before the invent of flash freezing, systematic errors due to
>>> decay and the need
>>> of scaling together many derivative data sets collected on
>>> multiple crystals could render
>>> weak anomalous signal useless. Therefore MIR was needed. Also,
>>> current hardware/software
>>> produces much better reduced data, so weak signals can become useful.
>>>
>>> Fred
>>>
>>> ?[32m*******************************************************************************
>>> Fred Dyda, Ph.D. Phone:301-402-4496
>>> Laboratory of Molecular Biology Fax: 301-496-0201
>>> DHHS/NIH/NIDDK e-mail:[log in to unmask]
>>> Bldg. 5. Room 303
>>> Bethesda, MD 20892-0560 URGENT message e-mail: [log in to unmask]
>>> Google maps coords: 39.000597, -77.102102
>>> http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred
>>> *******************************************************************************?[m
>
|