Hello,
Those min/max aliased frequencies are just used to set what you see on the
screen. Once you have done that the best way to proceed is to pick the
peak at the correct shift rather than the aliased shift. But if you have
already picked the peak at the wrong shift then you can fix that by
bringing up the right mouse menu Peak --> Unalias peak dialog and change
the Num. Aliasing there, or you can select the peak in the spectrum window
and hit "s" to bring up the Selected Peaks dialog, and then click on the
Unalias button at the bottom.
Wayne
On Mon, 25 Jun 2012, Pernille Møller wrote:
> How can I figure out to "set appropriately" to pick the peaks where they belong??
>
> I have a aliased peak at position (7.655;105.46) in my NH HSQC spectrum - I want to
> use my HNCA spectrum to link my amide from the NH-HSQC spectrum to a CAi and
> CAi-1 peak, but because my peak from NH HSQC is aliased I get two different N shifts
> for same amide (105.46 in my NH-HSQC and 127.50 in my HNCA (actual N-shift)).
>
> For my NH-HSQC spectrum I have:
>
> dim1 (1H); minimum aliased frequency (ppm): 4.766, maximum aliased frequency (ppm):
> 12.777
> dim2 (15N); minimum aliased frequency (ppm): 95.602, maximum aliased frequency (ppm):
> 135.602
>
> For my HNCA spectrum I have:
>
> Dim1 (1H); minimum aliased frequency (ppm):-1.738, maximum aliased frequency (ppm):
> 11.246
> dim2 (15N); minimum aliased frequency (ppm): 108.001, maximum aliased frequency (ppm):
> 130.001
> dim3 (13C); minimum aliased frequency (ppm): 38.001, maximum aliased frequency (ppm):
> 65.001
>
>
>
> Well, I don’t know how to set these parameters so that I get the actual N-shift
> (127.50) for my aliased peak?
>
> /Pernille
>
>
>
> > Date: Tue, 12 Jun 2012 14:40:19 +0100
> > From: [log in to unmask]
> > Subject: Re: Ambiguous peaks
> > To: [log in to unmask]
> >
> > Hello,
> >
> > On the second point (and hopefully Rasmus will comment on the first
> > point), you should pick the peaks at their actual shifts, not at their
> > folded (aliased) positions. You can set the region for display for a
> > given spectrum in the Experiment --> Spectra dialog in the Referencing
> > tab, in the Minimum/Maximum aliased frequency columns at the right. If
> > set appropriately then you can pick the peaks where they belong. Then the
> > program will know how to properly compare peaks. If you have a peak that
> > is currently in the incorrect location because of folding then you can fix
> > that with the right mouse menu Peak --> Unalias peak dialog, by changing
> > the "Num. Aliasing".
> >
> > Wayne
> >
> > On Tue, 12 Jun 2012, Pernille Møller wrote:
> >
> > > Hello CCPN
> > >
> > > I would like to know how you can assign a peak to be ambiguous. I have in my
> > > NH-HSQC spectrum a peak that has spin system i (H-N). In my HNCA spectrum I can
> > > find two CA's (they have equal size) but I dont know which one is from spin
> > > system i or i-1. So have can I assign these two CA peaks to be ambiguous (that
> > > they can be both possibilities, i and i-1)??
> > >
> > >
> > > Likewise if I have a peak that is folded how can I tell the program that the
> > > peak with two different Nitrogen shift is the same? I dont know where I can
> > > find a popup window for aliased peaks?
> > >
> > > /Pernille
> > >
> > >
>
>
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