Try adding DNA then dialyzing to low salt (in some microdialyer).
=====================================
Phoebe A. Rice
Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
http://www.rsc.org/shop/books/2008/9780854042722.asp
---- Original message ----
>Date: Mon, 18 Jun 2012 15:22:46 +0530
>From: CCP4 bulletin board <[log in to unmask]> (on behalf of Arpit Mishra <[log in to unmask]>)
>Subject: [ccp4bb] Fwd: Delivery Status Notification (Failure)
>To: [log in to unmask]
>
> Dear CCP4BB members
>
> Sorry for off topic, i had question related to
> dispersity of proteins of my
> interest, i am working on DNA binding protein,
> having PI around 9 to
> 10,Initially protein was remaining stable at high
> salt, but was not able to
> concentrate more than 1mg/ml, after using 50mm Arg,
> 50mm Glutamate i
> overcome the solubility and concentrating problem
> but problem now is that
> my protein is remaining polydisperse, so i want to
> try different
> additives, i had tried some detergents and
> chaotropic agent,pH ranges.but
> it didnt help, so any suggestions to bring my
> protein in monodisperse state
> that will be really helpful...thanks for your
> suggestions in advance
>
> --
> Arpit Mishra
>
>
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