I think some have used anomalous signals since the 1930s-40s, e.g., Bijvoet!
JPK
On Wed, Jun 6, 2012 at 10:23 AM, Ronald E Stenkamp
<[log in to unmask]> wrote:
> There were a number of labs using anomalous dispersion for phasing 40 years
> ago. The theory for using it dates from the 60s. And careful experimental
> technique allowed the structure solution of several proteins before 1980
> using what would be labeled now as SIRAS. Ron
>
>
> On Wed, 6 Jun 2012, Dyda wrote:
>
>>> I suspect that pure MIR (without anomalous) was always a fiction. I doubt
>>> that anyone has ever used it. Heavy atoms always give
>>> an anomalous signal
>>
>>
>>> Phil
>>
>>
>> I suspect that there was a time when the anomalous signal in data sets was
>> fictional.
>> Before the invent of flash freezing, systematic errors due to decay and
>> the need
>> of scaling together many derivative data sets collected on multiple
>> crystals could render
>> weak anomalous signal useless. Therefore MIR was needed. Also, current
>> hardware/software
>> produces much better reduced data, so weak signals can become useful.
>>
>> Fred
>>
>>
>> [32m*******************************************************************************
>> Fred Dyda, Ph.D. Phone:301-402-4496
>> Laboratory of Molecular Biology Fax: 301-496-0201
>> DHHS/NIH/NIDDK e-mail:[log in to unmask]
>> Bldg. 5. Room 303
>> Bethesda, MD 20892-0560 URGENT message e-mail: [log in to unmask]
>> Google maps coords: 39.000597, -77.102102
>> http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred
>>
>> *******************************************************************************
>> [m
>>
>
--
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
email: [log in to unmask]
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