Gene synthesis and make your own!
Tony.
Sent from my iPhone
On 24 May 2012, at 20:42, "Gloria Borgstahl" <[log in to unmask]> wrote:
> My fellow crystallographers,
> We are thinking the SUMO/His vectors would be nice to have in the lab
> aresenal... but. The stumbling block is that the protease needed for
> cleavage is very expensive at crystallography scale. SUMO(ULP-1)
> protease costs ~$700/mg fusion protein. It would not be a problem
> for labs using micrograms of protein, but is prohibitive at our level
> of protein purification.
> Has anyone found a way around this? Your pal, Gloria
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