Dear community,
I am trying to cleave a hexaHis tag from my protein prior to crystallization.
As I was setting up my digestion, my protein started to precipitate as soon as I added the recommended thrombin buffer.
My question is, if anyone has encountered this, how well does it cleave without thrombin buffer?
or even, without the CaCl2? Any other buffer/conditions known to work?
thanks in advance
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