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Dear Theresa,
I was not aware you need DTT for TEV protease activity. People do
on-column digestion, and as far as I remember, a Ni-column would turn
really uglily brown of you used DTT on those columns.
Have you tried to leave out DTT and the cleavage did not work?
Cheers,
Tim
On 04/16/12 09:31, Theresa Hsu wrote:
> Dear all
>
> I want to digest a tagged protein with TEV protease, it has
> disulfide bridges. Is there any way of doing cleavage without DTT?
>
> Thank you.
>
> Theresa
>
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
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