I am using KPi buffer at pH 5.5, 100mM KCl, 2mM beta-mercaptoethanol, 0.02%
Yes, I agree I should check CD melting curve to see temperature preference
of my protein.
| -----Original Message-----
| From: Kevin Jin [mailto:[log in to unmask]]
| Sent: Monday, March 12, 2012 11:16 AM
| To: Min-Kyu Cho
| Cc: [log in to unmask]
| Subject: Re: [ccp4bb] My protein precipitates at r.t and dissolves at 4
| Which kind of buffer you use? If it is Tris, then temperature change will
| cause pH change.
| Actually, this is a good way for crystallization.
| On Mon, Mar 12, 2012 at 9:02 AM, Min-Kyu Cho <[log in to unmask]>
| > Hi all,
| > I have a homotetrameric coiled-coil domain sample with 45aa per each.
| > While I store this sample at 4oC, the sample looks clear w/o any
| > particles. But when I took out the sample to my bench at r.t, I can
| > see there are precipitates (as stack of needle like particles) at the
| > bottom of the tube after several hours. Interestingly, when I put it
| > back into 4oC fridge, the precipitates disappeared and the solution
| turned into clear again.
| > Does anyone have knowledge of such behavior of any protein? I
| > appreciate any information related.
| > Min-Kyu