I guess you already run SDS-PAGE to check the pellets before and after
sonication. Not only the media.
On Tue, Mar 27, 2012 at 7:35 AM, rana ibd <[log in to unmask]> wrote:
> Dear all
> I am expressing a 6xHis tagged in a dHBx protein in E.coli BL21 using LB
> madia, I am having problems with the expression which shows small amount of
> the protein , I also have problems with purification using NI-NTA by also
> having small amount even after extensive buffer exchange , Is it likely due
> to the small amount of protein in the medium , should I use a different kind
> of media, any sugestions or any kind of details or a paper that might help I
> will be thankful
> Best Regards
Candle always burns itself out to light up the tunnel .....