Rationalising it completely may only be possible once you know the nature of the crystal contacts, i.e. when you have solved the structure. Until then it is mainly a matter of experimenting.
>
> -----Original Message-----
> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
> Theresa H. Hsu
> Sent: Monday, February 06, 2012 11:00 PM
> To: [log in to unmask]
> Subject: Re: [ccp4bb] Freezing crystal
>
> Hi all
>
> Thanks for all the suggestions which I will try soon.
>
> How do the crystallization condition (PEG vs. salts like ammonium
> sulfate) affect the croyprotectant condition? Do factors like presence
> of low concentration of high molecular weight PEG (> 2000) mean PEG is
> better? Do buffers and salts in protein also important?
>
> Trying to rationalize it :)
>
> Theresa
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