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Subject:
Re: about point mutation
From:
D Bonsor <[log in to unmask]>
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Date:
Fri, 24 Feb 2012 14:34:29 +0000
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text/plain
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Phusion requires that the primers are phosphorylated for mutagensis to work, unlike Pfu. If you cannot phosphorylate them use Pfu as recommended by Charlotte Dan
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