hi
did u have other crystals grew in different conditions in your additive screen ?
in my case, i did the additve screen for the condition i got twinned crystals
i got crystals grew in few conditions
one of them is salt but it still gave me twinned crystals
then i tried NDSB-221 and the crystals turned out non-twin
hope it helps
by the way, even though its twinned crystal
should be ok to solve the structure
Mary
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From: CCP4 bulletin board [[log in to unmask]] on behalf of Francis E Reyes [[log in to unmask]]
Sent: Thursday, February 23, 2012 4:06 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] effects of salt on twinned crystals
I read your description of the crystals and Figure 4 of the following paper came to mind. Post-crystallization treatment in lower PEG eventually allowed them to tease the bundles apart.
1. MacRae, I. J. & Doudna, J. A. An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer. urn:issn:0907-4449 63, 993–999 (2007).
F
On Feb 22, 2012, at 10:59 AM, Peter Hsu wrote:
> Forgot to mention, that this 2.5-3A diffracting crystal was the same one that I have been unable to index and suspect are twinned due to the presence of these other fused crystals in the same drop.
>
> Thanks for any input.
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Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
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