This might be obvious, but make sure you washed the crystals
thoroughly before dissolving them for SDS-PAGE or mass spec
-Jesse
On Thu, Jan 26, 2012 at 10:48 AM, Katherine Sippel
<[log in to unmask]> wrote:
> Might I suggest consulting the CCP4 user community wiki on the topic:
>
> http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Improving_crystal_quality
>
> Good luck,
>
> Katherine
>
>
>
> On Thu, Jan 26, 2012 at 9:33 AM, Theresa H. Hsu <[log in to unmask]>
> wrote:
>>
>> Dear crystallographers
>>
>> I have a protein of 90 kDa forming dimers. Crystals formed with microbatch
>> and vapor diffusion method in 24 hours but no diffraction at home source.
>> Dissolved crystals was confirmed to be the protein with mass spec.
>>
>> Any suggestions to improve diffraction would be welcome.
>>
>> Thanking you in advance.
>>
>> Theresa
>
>
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