Are you close to the theoretical isoelectric point of your protein ? Change pH of buffer
Jürgen
......................
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-3655
http://web.mac.com/bosch_lab/
On Jan 28, 2012, at 10:55, "rashmi panigrahi" <[log in to unmask]> wrote:
> Hi all,
> I tried to concentrate my protein using vivaspin 20 10,000 MWCO PES.
> The protein was in 50mM Hepes pH 7.5, 500mM KCl and 10% glycerol.
> I lost about 90% of my protein on the membrane of the centricon.
>
> Please suggest some way of concentrating this protein.
> Will concentrating using peg 20K be a good alternative??
>
> regards
>
> rashmi
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