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CCP4BB December 2011
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Subject:
Expression of a Selenomethionine Variant in E. coli
From:
Yibin Lin <[log in to unmask]>
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Date:
Mon, 12 Dec 2011 22:56:40 +0100
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text/plain
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text/plain (11 lines)
Hi List, I want to preprare sel-met labeling protein. Could someone can tell me if it is necessary to add DTT or BME to medium, which contains sel-met, when growing cells? Thanks in advance, Yibin Lin
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