Tendamistat (1OK0) was crystallized at pH 1.3 and diffracted to 0.93A.
George
On Mon, Nov 07, 2011 at 05:19:29AM +0000, Sam Arnosti wrote:
> Hi everyone
>
> I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
>
> I want to crystallize it in the low PH and compare the differences between the crystals in regular PH and low PH.
>
> I was wondering how people set up the boxes in low PH, as usual buffers are mostly less acidic.
>
> Regards
>
> Sam
>
--
Prof. George M. Sheldrick FRS
Dept. Structural Chemistry,
University of Goettingen,
Tammannstr. 4,
D37077 Goettingen, Germany
Tel. +49-551-39-3021 or -3068
Fax. +49-551-39-22582
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