Hi Len;
I was having exactly the same problem with my crystals, but when we grow
the crystals in presence of increasing concentration of Glycerol and MPD
starting from 0.5 to 10%. The crystal doesn't appear after 3% of Glycerol
or MPD but the one which appear in 2.5 to 3 % were much resistant to
cracking than the original crystals.
Good luck
Bashir
On Wed, October 26, 2011 18:46, Leonard Thomas wrote:
> Hi All,
>
> I have run into a very sensitive crystals system when it comes to cryo
> protecting them. I have run through the usual suspects and trays are
> going to be setup with a cryo protectant as part of crystallization
> cocktail. The one problem that seems to be occurring is that the
> crystals crack as soon as they are transfered out of the original
> drop. I am running out of ideas and really would love some new ones.
>
> Thanks in advance.
>
> Len
>
> Leonard Thomas Ph.D.
> Macromolecular Crystallography Laboratory Manager
> University of Oklahoma
> Department of Chemistry and Biochemistry
> Stephenson Life Sciences Research Center
> 101 Stephenson Parkway
> Norman, OK 73019-5251
>
> [log in to unmask]
> http://barlywine.chem.ou.edu
> Office: (405)325-1126
> Lab: (405)325-7571
>
--
Muhammad Bashir Khan
**************************************************
Department for Structural and Computational Biology
Max F. Perutz Laboratories
University of Vienna
Campus Vienna Biocenter 5
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Austria
Austria
Phone: +43(1)427752224
Fax: +43(1)42779522
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