dear SnowDeer,
The first step to see if bigger crystals can be grown is to use bigger
protein drops and vary
the precipitant/protein ratio at the beginning of the vapour diffusion
experiment.
You can work out for yourself why this
should give you all the informations that you need in subsequent
experiments.
Enrico.
On Mon, 05 Sep 2011 10:06:22 +0200, SnowDeer <[log in to unmask]> wrote:
> Dear All:
>
> Recently I am working on a protein which can already grow nice
> pyramid-like
> crystals after the condition was optimized, while the crystals are too
> small
> to be picked up. The crystals grew quite fast and densely, so I tried to
> put
> 100ul paraffin oil inside the 600ul reservoir solution or put the plate
> under 16 degree to slow down the evaporation, while the crystals were
> still
> the same. I also tried macro or micro seeding with or without the
> paraffin
> oil. Macroseeding would give a larger crystal (not very nice) with many
> small crystals in the drop even I washed the seeds carefully. For
> microseeding, the same small crystals grew.
>
> I don't have many experiences in crystallography, so I have no idea how
> to
> make it grow bigger...
>
> Any suggestion is most welcome.
>
> Thanks.
> SnowDeer
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: [log in to unmask] Fax: 33 (0)1 69 08 90 71
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