i wonder if "crushing with a hammer" maybe a good first step. does
streak seeding after that give smaller crystals amongst the range of
different size crystals possible. perhaps? either way would be good
for the summer student to try out.
--
Karthik
On Tue, Jul 26, 2011 at 10:55 AM, James Holton <[log in to unmask]> wrote:
> Does anyone out there have a protocol of growing HEWL crystals that are all
> 50-100 microns wide? I gave this project to a summer student recently,
> thinking it would be easy, but it is turning out to be more difficult than I
> thought. Keep getting sphereulites instead of small crystals. Yes, I know
> you can smash a large lysozyme crystal with a hammer, but that is not
> exactly what I was going for. What I was hoping for was a well-defined
> protocol for growing "reference" crystals that stay evenly illuminated in
> our x-ray beams as they rotate. The beam is 100 um wide.
>
> I'm sure someone has done this before?
>
> -James Holton
> MAD Scientist
>
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