On 06/08/2011 07:19 PM, Shiva Bhowmik wrote:
> Dear All,
>
> I am working on a protein structure that yielded comparable diffraction
> quality crystals from two different crystallization condition. One of the
> crystallization condition conatins high conc. of salt pptant whereas the
> oher one contains high conc. of organic pptant. There are some minor
> differences in the stucture with respect to the backbone but what most
> surprising is the oligomeric structure. AnSEC study suggest the protein to
> be a tetramer in solution and a tetrameric assembly is observed in the
> asymmetric unit of the space group of the crystal obtained from high conc.
> of organic pptant. However, the crystal structure from the high conc. of
> salt pptant does not reveal any oligomeric assembly - symmetry operations of
> the space group does not suggest any oligomeric assembly. I believe the high
> salt conc. disrupted the tetrameric assembly and enabled crystallization of
> the protein as a monomer.
>
> Curious to know if there has been any similar precedence before.
>
> Cheers,
>
> Shiva
>
I would submit both structures to PISA at the EBI, and look at the
interfaces to see if there is any similarity between the 2 forms..
Eleanor
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