Never had problems with evaporation (and this is in the relatively dry
climate of Denver, CO, especially in the winter when the relative
humidity is in the low 20%).
Using the Thermo Scientific Nanodrop 2000c.
We use it also as a prerequisite for ITC, which can be very sensitive
to proper concentrations.
F
On Jun 16, 2011, at 1:15 PM, Arnon Lavie wrote:
> Dear fellow crystallographers - a question about spectrophotometers
> for protein concentration determination.
>
> We are so last millennium - using Bradford reagent/ 1 ml cuvette for
> protein conc. determination.
>
> We have been considering buying a Nanodrop machine (small volume, no
> dilution needed, fast, easy).
> However, while testing our samples using a colleague's machine, we
> have gotten readings up to 100% different to our Bradford assay (all
> fully purified proteins). For example, Bradford says 6 mg/ml,
> Nanodrop 3 mg/ml. So while it is fun/easy to use the Nanodrop, I am
> not sure how reliable are the measurements (your thoughts?).
>
> So QUESTION 1: What are people's experience regarding the
> correlation between Nanodrop and Bradford?
>
> While researching the Nanodrop machine, I heard about the Implen
> NanoPhotmeter Pearl.
> So Question 2: Is the Pearl better/worse/same as the Nanodrop for
> our purpose?
>
> Thank you for helping us to advance to the next millennium, even if
> it is nearly a dozen years late.
>
> Arnon
>
> --
> ***********************************************************
> Arnon Lavie, Professor
> Dept. of Biochemistry and Molecular Genetics
> University of Illinois at Chicago
> 900 S. Ashland Ave.
> Molecular Biology Research Building, Room 1108 (M/C 669)
> Chicago, IL 60607
> U.S.A.
> Tel: (312) 355-5029
> Fax: (312) 355-4535
> E-mail: [log in to unmask]
> http://www.uic.edu/labs/lavie/
> ***********************************************************
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