Thanks a lot! I did find some map statistics (average, standard
deviation...). Tt also provide a map dimension, where I think we can
covert the contour level to X-ray sigma level.
Thanks again for the information.
Hailiang
> Try here:
> http://www.ebi.ac.uk/pdbe/emdb/
> You can (if you get the EM map) play around with various programs and
> manipulate the level until the mask matches the molecular weight of your
> object of interest.
> Or contact the authors of the EM map. Ask for either CCP4 format map or
> MRC, you can use programs from the USF suite to inter convert between the
> formats. If they used EMAN then most likely the CCP4 format is still bogus
> but MRC works fine.
>
> Jürgen
>
> On Jun 2, 2011, at 5:24 PM, Hailiang Zhang wrote:
>
> Hi,
>
> I am trying to compare a published EM map with X-ray map in hand, and have
> several questions:
>
> 1. EM map seldom indicates the sigma level, and it was said because of the
> box size uncertainty during EM model construction. Now, I wonder is there
> any way we can sort of its equivalent sigma level to X-ray map?
>
> 2. The EM structure deposited in pdb don't have any experimental data, and
> not sure how to obtain them and generate the map file.
>
> Sorry if this is wrong place to post EM questions.
>
> Hailiang
>
> ......................
> Jürgen Bosch
> Johns Hopkins Bloomberg School of Public Health
> Department of Biochemistry & Molecular Biology
> Johns Hopkins Malaria Research Institute
> 615 North Wolfe Street, W8708
> Baltimore, MD 21205
> Phone: +1-410-614-4742
> Lab: +1-410-614-4894
> Fax: +1-410-955-3655
> http://web.mac.com/bosch_lab/
>
>
>
>
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