I think you can get salt crystals from MgCitrate as well, so be forewarned!
Jacob
On Mon, Jun 20, 2011 at 6:55 PM, Nat Echols <[log in to unmask]> wrote:
> On Mon, Jun 20, 2011 at 4:09 PM, Jayashankar <[log in to unmask]>
> wrote:
>>
>> Should I be happy if i get my crystals with one of the condition with a
>> commercial kit
>> containing citric acid and 40%MPD+ protein in(hepes+MgCl2)+ADP+VO3
>
> Citric acid will chelate magnesium - this may not disrupt the
> crystallization of the protein, but it could result in a non-physiological
> active site. The coordination geometry of Mg2+ is hard to miss, so it's
> usually obvious whether it's there or not despite being the same number of
> electrons as water, but if you don't see a nice hexahedral arrangement, the
> citrate took it all.
> -Nat
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
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