Hi Wayne
I have tried it with and without those options selected but i still
get the same result, is there a reason for this?
On May 26, 2011, at 6:12 PM, Wayne Boucher wrote:
> Hello,
>
> Tim suggests that you should click the "Pick new peaks" and "Pick
> non-maxima peaks" check boxes. Now what that does is that it will
> try and find a local extremum within the specified tolerances and if
> it doesn't find one it will put down a peak at the reference
> position. So if you want all your peaks to be at the reference
> position then you should make the tolerances small enough that it
> will then not find any extrema. The tolerances are set in the table
> below those two check boxes, you might need to resize the dialog to
> see it.
>
> Wayne
>
> On Thu, 26 May 2011, S.P. Skinner wrote:
>
>> Hi All
>>
>> I am using the follow intensity changes function to calculate R1
>> and R2 rates for my protein, but there appears to be an issue
>> wherein the peak position changes from point to point. I have
>> picked and assigned the first plane, but not the others. Is there
>> a way to force the peak picking to stay in exactly the same place
>> or would I have to manually assign all planes of the pseudo 3D
>> spectra? If the latter is true, is there a quick way to do this?
>>
>> Regards
>>
>> Simon
>>
>> --
>> Simon P Skinner
>> Protein Chemistry Group
>> Leiden Institute of Chemistry, Universiteit Leiden
>> Phone: +31 71 527 6089 / Fax: +31 71 527 4349
>> E-mail : [log in to unmask]
>>
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