[Of course it might be worth saying that if your peaks are moving around
then you might have 'issues' with RF sample heating or at least
temperature regulation. If your peaks move more in the R2 experiment
than in the R1 experiment and they move systematically with increasing
CPMG duration then you might need to worry about the accuracy of your R2
values. Only trying to help.]
Paul
On 26/05/2011 17:12, Wayne Boucher wrote:
> Hello,
>
> Tim suggests that you should click the "Pick new peaks" and "Pick
> non-maxima peaks" check boxes. Now what that does is that it will try
> and find a local extremum within the specified tolerances and if it
> doesn't find one it will put down a peak at the reference position.
> So if you want all your peaks to be at the reference position then you
> should make the tolerances small enough that it will then not find any
> extrema. The tolerances are set in the table below those two check
> boxes, you might need to resize the dialog to see it.
>
> Wayne
>
> On Thu, 26 May 2011, S.P. Skinner wrote:
>
>> Hi All
>>
>> I am using the follow intensity changes function to calculate R1 and
>> R2 rates for my protein, but there appears to be an issue wherein the
>> peak position changes from point to point. I have picked and
>> assigned the first plane, but not the others. Is there a way to
>> force the peak picking to stay in exactly the same place or would I
>> have to manually assign all planes of the pseudo 3D spectra? If the
>> latter is true, is there a quick way to do this?
>>
>> Regards
>>
>> Simon
>>
>> --
>> Simon P Skinner
>> Protein Chemistry Group
>> Leiden Institute of Chemistry, Universiteit Leiden
>> Phone: +31 71 527 6089 / Fax: +31 71 527 4349
>> E-mail : [log in to unmask]
>>
--
Paul Driscoll
Group Leader
Division of Molecular Structure
MRC National Institute for Medical Research
Tel. +44 (0)20 8816 2061 / +44 (0)7876 777937
http://www.nimr.mrc.ac.uk/research/paul-driscoll
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