Dear all,
I am considering trying to crystallize a small peptide (around 15 amino acids). The peptide is soluble in neutral water or buffer (pH 7.0) until at least 10 mM (13 mg/mL), and adopts a turn conformation when bound to Zn.
What are your thoughts on attempting this?
If you think that it is worthwhile, what crystallization conditions would you try? I am thinking of a sparse matrix screen using the Hampton Crystal Screen 1 and 2 kits, using hanging drop crystallization in Hampton Vdx trays.
Thanks! and all the best,
---Buz
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