You probably use hanging drops. It's the surface tension effect. Check
if sitting drops are better.
Maia
Sitting drops
weikai wrote:
> Hi Folks,
>
> We have some membrane protein crystals that are grown in 30%PEG400,
> 0.1M Na Citrate pH 4.5, 0.1M LiCl. The protein is purified in DDM. The
> crystals are long rods and grown under room temperature in a hanging
> drop set up. But once we open the cover slip, we see the rods start
> to break and bend in a few seconds. Since it is in high PEG400, we
> just directly freeze the crystals. The diffraction only goes to 10
> Ang at synchrotron. Have anybody had similar problem before and any
> suggestions?
>
> Thanks a lot,
>
> Weikai
>
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